ABSTRACT
Objective@# To investigate the awareness of monkeypox prevention knowledge and its influencing factors among inbound personnel, so as to provide insights into intensified health education for monkeypox prevention and control.@*Methods@#Inbound personnel at ages of 16 years and older were sampled using a convenient sampling method from centralized medical isolation observation points in Gongshu District, Hangzhou City in November and December 2022. Participants' demographic features, history of travel in countries where monkeypox cases were reported and awareness of monkeypox prevention knowledge were collected through questionnaire surveys, and factors affecting the awareness of monkeypox prevention knowledge were identified using a multivariable logistic regression model. @*Results@#A total of 306 questionnaires were allocated, and 293 valid questionnaires were recovered, with an effective response rate of 95.75%. The respondents included 192 men (65.53%) and 101 women (34.47%), and hand a median (interquartile range) age of 36 (16) years. The overall awareness of monkeypox prevention knowledge was 44.71%, and the awareness rates of preventive measures, source of infection, transmission route, susceptible populations and clinical manifestations were 82.94%, 79.18%, 75.09%, 60.75% and 60.07%, respectively. Multivariable logistic regression analysis showed that workers (OR=0.179, 95%CI: 0.040-0.800), self-employed individuals (OR=0.090, 95%CI: 0.018-0.451), and those with other occupations (OR=0.157, 95%CI: 0.034-0.728) had lower awareness of monkeypox prevention knowledge than students, and individuals who had never heard of monkeypox (OR=0.056, 95%CI: 0.007-0.447) had lower awareness of monkeypox prevention knowledge than those who had heard of it. In addition, individuals who were not concerned about monkeypox epidemics (OR=0.048, 95%CI: 0.004-0.563) had lower awareness than those who were very concerned. @*Conclusions@#Inbound personnel have low awareness of monkeypox prevention knowledge, and occupation, having heard of monkeypox and concern about monkeypox epidemics may affect the awareness of monkeypox prevention knowledge. Intensified health education about monkeypox is required to improve the preventive awareness among inbound personnel.
ABSTRACT
Exosomes, a kind of extracellular vesicle, are promising therapeutic agents for spinal cord injury (SCI). This article aimed to investigate effects of exosomes secreted from miRNA-29b-modified bone marrow mesenchymal stem cells (BMSCs) on SCI. Exosomes were extracted from BMSCs transfected with miRNA-29b or negative control (miR NC). SCI rats were injected intravenously with exosomes (control exosomes, miRNA-29b exosomes) and BMSCs (miR NC, miRNA-29b) through the tail vein. The expression of miRNA-29b in spinal cord tissues of SCI rats was detected by qRT-PCR. The hind limb motor function was evaluated by Basso Beattie Bresnahan (BBB) score. The histopathological damage and neuronal regeneration in spinal cord tissues was observed by HE staining and immunohistochemistry, respectively. The injection of miRNA-29b exosomes and miRNA-29b BMSCs both significantly increased the expression of miRNA-29b in spinal cord tissues of SCI rats (P<0.05). Compared with SCI rats, rats in the miRNA-29b exosomes and the miRNA-29b groups exhibited improved SCI, including increased BBB score, NF200 and GAP-43 positive neurons, as well as decreased contractile nerve cell numbers and GFAP positive neurons (all P<0.05). The relieving degree of SCI was significantly higher in the miRNA-29b exosomes group than in the miRNA-29b BMSCs group (P<0.05). Exosomes secreted from miRNA-29b-modified BMSCs were effective in the repair of SCI in rats.